细胞培养技术及货期价格相关可联系客服 |
QQ784129740 微信18717770857 |
生长特性: |
混合生长 |
形态特征 |
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培养条件: |
培养基:ACL-4 medium (serum-free)The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:0.02 mg/ml insulin0.01 mg/ml transferrin25 nM sodium selenite (final conc.)50 nM Hydrocortisone (final conc.)1 ng/ml Epidermal Growth Factor (do not filter)0.01 mM ethanolamine (final conc.)0.01 mM phosphorylethanolamine (final conc.)100 pM triiodothyronine (final conc.)0.5% (w/v) bovine serum albumin (final conc.)0.5 mM sodium pyruvate (final conc.)extra 2mM L-glutamine (for final conc. of 4.5mM)
气相:空气 95%,二氧化碳 5%
温度:37℃ |
简介: |
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细胞总数: |
1~3*106 |
传代周期: |
2-3 天 |
传代方法: |
1:5-1:8传代; |
传代比例: |
1:2~1:4传代 |
换液频率: |
2-3 天 |
冻存液: |
无血清细胞冻存液 |
STR: |
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