中文名称

正常人结肠上皮细胞；FHC

组织

结肠

形态特性

上皮样

生长特性

贴壁生长

背景特征

Characteristics: Forms solid tumors after transplantation into SCID mice.Doubling time: ~62 hours (PubMed=6519668).Karyotypic information: Is hypotriploid， has >20 copies of the 8q23~8q24.3 region containing MYC.Omics: Transcriptome analysis.Derived from sampling site: Fetal colon.

培养体系

he base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium， add the following components to the base medium:extra 10 mM HEPES (for a final conc. of 25 mM)10 ng/ml cholera toxin0.005 mg/ml insulin0.005 mg/ml transferrin100 ng/ml hydrocortisone20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)Fetal Bovine Serum， 10% final conc (ATCC 30-2020)

培养条件

空气，95%；CO2，5%。 温度：37℃

传代方法

建议第一次1:2传代   换液频率2~3次/周

冻存条件

90FBS+10%DMSO,推荐无血清冻存液（CX001）

产品使用

仅限于科学研究